Cisplatin

The cisplatin Structure.
The retention Mechanism
the stability of Cis-DDP: Cis platin is known for unstable condition in aqueous solutions but when increasing the chloride concentration Cis-DDP will be more stable. Read more at the stability of cisplatin in sodium chloride and ammonium chloride. The concentration of chloride range 4-20mM in favor for the hydrolysis.
Standards are analyzed when diluted in different solution, water, ammonium chloride 50mM, and sodium chloride.
Cisplatin is a polar compound so it can be isolated by hydrophilic colum, Zic-hilic coloum is employed for this perpose.
- CDDP is hydrolyzed in water


in water MH-CDDP is formed, the half life formation of MH-CDDP is around 120min
in cell extract the half-life of cisplatin is 75 min
of cisplatin mono-hydrated derivatives binding to DNA, causing cell death
(interaction with DNA,