GHS-CDDP

in vitro adducts include: G1-G11
1mM CDDP + 10mM GSH (10/1, v/v)----> 0.9mM CDDP and 0.9 mM GSH then incubated in 1,3,4,5,6, 24 hours.
the detection of GSH-CDDP is performed by a reversed phase column.
Eluent: 15mM HCOOH in 90/10/methnol, optimum flowrate 1mL/min
UV detector.