Electrospray (ESI) and applitcation

For the detection of proteins, polymers, biopolymers, polar molecules.
offer high sensitivity, easy to be coupled with HPLC.





Mass spectrometry - principles and applications. p.40

Applied voltage: 3-6 kV
Onset voltage: 4 kV for water (surface tension 0.073N/m2)
                          2.2 kV for methanol (surface tenstion 0.023Nm−2)
                          2.5 kV for acetonitrile (surface tension 0.030Nm−2). 
                          3 kV for dimethylsulfoxide (0.043Nm−2) 
z1*m1 = M + z1*mP
z1 = positive charge ion
m1 = mass/charge
mP = mass of proton
M = molecule weight.